| Professional Development League | - | 2024-05-07 20:00 | Queens Park Rangers U21 | | Barnsley U21 | |
| Professional Development League | - | 2024-05-03 18:00 | Barnsley U21 | 2-0 | Hull City U21 | 1-0 |
| Professional Development League | - | 2024-04-23 21:00 | Millwall U21 | 3-3 | Barnsley U21 | 1-3 |
| Professional Development League | - | 2024-04-19 20:00 | Burnley U21 | 0-4 | Barnsley U21 | 0-2 |
| Professional Development League | - | 2024-04-16 20:00 | Barnsley U21 | 4-1 | Bristol City U21 | 1-1 |
| Professional Development League | - | 2024-04-10 21:00 | Charlton Athletic U21 | 0-4 | Barnsley U21 | 0-1 |
| Professional Development League | - | 2024-03-26 21:00 | Barnsley U21 | 1-2 | Watford U21 | 0-1 |
| Professional Development League | - | 2024-03-19 21:00 | Barnsley U21 | 3-1 | Coventry City U21 | 1-0 |
| Professional Development League | - | 2024-03-14 03:00 | Birmingham City U21 | 3-1 | Barnsley U21 | 1-0 |
| Professional Development League | - | 2024-03-07 23:00 | Barnsley U21 | 4-2 | Wigan Athletic U21 | 1-0 |
| Professional Development League | - | 2024-02-27 22:00 | Peterborough United U21 | 2-2 | Barnsley U21 | 0-2 |
| Professional Development League | - | 2024-02-20 21:00 | Barnsley U21 | 1-2 | Sheffield Wednesday U21 | 0-1 |
| Professional Development League | - | 2024-02-14 03:00 | Sheffield United U21 | 0-1 | Barnsley U21 | 0-0 |
| Professional Development League | - | 2024-01-23 21:30 | Sheffield Wednesday U21 | 1-0 | Barnsley U21 | 1-0 |
| Professional Development League | - | 2024-01-16 21:00 | Barnsley U21 | | Burnley U21 | |
| Professional Development League | - | 2024-01-09 21:00 | Fleetwood Town U21 | 2-4 | Barnsley U21 | 1-3 |
| Professional Development League | - | 2023-12-22 20:00 | Barnsley U21 | 3-0 | Crewe Alexandra U21 | 0-0 |
| Professional Development League | - | 2023-12-18 21:00 | Crewe Alexandra U21 | 1-2 | Barnsley U21 | 0-0 |
| Professional Development League | - | 2023-12-05 21:00 | Barnsley U21 | 1-2 | Birmingham City U21 | 0-0 |
| Professional Development League | - | 2023-11-07 21:00 | Coventry City U21 | 0-2 | Barnsley U21 | 0-2 |
| Professional Development League | - | 2023-10-30 21:00 | Wigan Athletic U21 | 5-0 | Barnsley U21 | 4-0 |
| Professional Development League | - | 2023-10-24 20:00 | Barnsley U21 | 3-2 | Fleetwood Town U21 | 2-1 |
| Professional Development League | - | 2023-10-18 02:00 | Hull City U21 | 3-1 | Barnsley U21 | 1-0 |
| Professional Development League | - | 2023-10-03 20:00 | Barnsley U21 | 4-2 | Peterborough United U21 | 0-1 |
| Professional Development League | - | 2023-09-28 02:30 | Barnsley U21 | 0-0 | Sheffield United U21 | 0-0 |
| Professional Development League | - | 2023-09-12 20:00 | Barnsley U21 | 1-3 | Ipswich Town U21 | 0-2 |
| Professional Development League | - | 2023-09-01 21:00 | Cardiff City U21 | 2-1 | Barnsley U21 | 0-0 |
| Professional Development League | - | 2023-08-29 20:00 | Barnsley U21 | 3-0 | AFC Bournemouth U21 | 1-0 |
| Professional Development League | - | 2023-08-21 21:00 | Colchester United U21 | 3-2 | Barnsley U21 | 2-0 |
| Professional Development League | - | 2023-08-15 20:00 | Barnsley U21 | 4-2 | Swansea City U21 | 3-2 |
| Professional Development League | - | 2023-05-05 20:00 | Reading U21 | 0-3 | Barnsley U21 | 0-2 |
| Professional Development League | - | 2023-05-02 19:00 | Barnsley U21 | 1-1 | Queens Park Rangers U21 | 0-1 |
| Professional Development League | - | 2023-04-25 21:00 | Cardiff City U21 | 3-1 | Barnsley U21 | 1-1 |
| Professional Development League | - | 2023-04-18 20:00 | Barnsley U21 | 2-1 | Swansea City U21 | 0-1 |
| Professional Development League | - | 2023-04-11 20:30 | Peterborough United U21 | 2-1 | Barnsley U21 | 0-0 |
| Professional Development League | - | 2023-04-07 20:00 | Watford U21 | 0-2 | Barnsley U21 | 0-0 |
| Professional Development League | - | 2023-04-05 02:00 | Colchester United U21 | 1-1 | Barnsley U21 | 0-0 |
| Professional Development League | - | 2023-03-28 19:00 | Sheffield Wednesday U21 | 0-1 | Barnsley U21 | 0-0 |
| Professional Development League | - | 2023-03-14 21:00 | Barnsley U21 | 3-0 | Burnley U21 | 1-0 |
| Professional Development League | - | 2023-03-03 21:00 | Sheffield United U21 | 2-1 | Barnsley U21 | 0-1 |
| Professional Development League | - | 2023-02-21 21:00 | Barnsley U21 | 1-1 | Coventry City U21 | 1-1 |
| Professional Development League | - | 2023-02-14 03:00 | Hull City U21 | 3-0 | Barnsley U21 | 3-0 |
| Professional Development League | - | 2023-02-07 21:00 | Barnsley U21 | 2-1 | Hull City U21 | 1-0 |
| Professional Development League | - | 2023-01-31 22:00 | Barnsley U21 | 1-0 | Wigan Athletic U21 | 0-0 |
| Professional Development League | - | 2023-01-06 21:00 | Barnsley U21 | 1-2 | Birmingham City U21 | 1-0 |
| Professional Development League | - | 2022-12-05 21:00 | Crewe Alexandra U21 | 3-1 | Barnsley U21 | 0-1 |
| Professional Development League | - | 2022-11-14 22:00 | Burnley U21 | 3-0 | Barnsley U21 | 2-0 |
| Professional Development League | - | 2022-11-07 21:00 | Wigan Athletic U21 | 1-6 | Barnsley U21 | 1-3 |
| Professional Development League | - | 2022-11-01 21:00 | Barnsley U21 | 3-0 | Sheffield United U21 | 2-0 |
| Professional Development League | - | 2022-10-21 20:00 | Barnsley U21 | 1-2 | Crewe Alexandra U21 | 1-1 |
| Professional Development League | - | 2022-10-17 20:30 | Birmingham City U21 | 1-1 | Barnsley U21 | 1-0 |
| Professional Development League | - | 2022-10-07 20:00 | Barnsley U21 | 1-2 | Sheffield Wednesday U21 | 1-1 |
| Professional Development League | - | 2022-09-30 20:00 | Coventry City U21 | 2-0 | Barnsley U21 | 1-0 |
| Professional Development League | - | 2022-09-27 21:00 | Barnsley U21 | 1-1 | Peterborough United U21 | 0-0 |
| Professional Development League | - | 2022-09-02 21:00 | Barnsley U21 | 2-0 | Charlton Athletic U21 | 2-0 |
| Professional Development League | - | 2022-08-23 21:00 | Barnsley U21 | 2-2 | Ipswich Town U21 | 1-2 |
| Professional Development League | - | 2022-08-16 20:00 | Millwall U21 | 4-0 | Barnsley U21 | 2-0 |
| Professional Development League | - | 2022-08-09 21:00 | Barnsley U21 | 2-3 | Bristol City U21 | 1-2 |
